Conjugation of curcumin and gold with green chemistry is an approach to improve the\neffectiveness of curcumin as anti-fibrosis. In this work, curcumin and gold were conjugated to deliver\ncurcumin to the liver. Curcumin-gold nanoparticles (cAuNPs) were prepared by varying curcumin\npH and concentration. The successful of cAuNPs formation were identified by using UV-visible\nand FTIR spectrophotometers. The particle size and morphology were analyzed using particle size\nanalyzer and cryo-TEM respectively. In vitro antioxidant assay was performed to determine the\ncurcumin activity after conjugation. Physical and chemical stabilities of cAuNPs were studied for\none month at 5 C, 25 C, and 40 C. Furthermore, the cAuNPs activity to modulate early marker of\nfibrosis was tested on NIH/3T3 cells. The optimum condition for cAuNPs synthesis was by using\n1.5 mM curcumin at pH 9.3. As compared to free curcumin, cAuNPs showed higher antioxidant\nactivity and maintained the nanosize after stored for one month. In line with the antioxidant activity,\ncAuNPs 0.25â??1.... reduced the collagen production by NIH/3T3 cells. More importantly,\ncAuNPs did not demonstrate any effect on the development of chicken embryo. Taken together,\nthe attachment of gold to curcumin in the form of cAuNPs is promising for curcumin targeting to\ntreat hepatic fibrosis.
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